Наукові журнали та збірники видань
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Документ The structure and topography of lymphoid tissue in immune formations of intestines in ducks(ВЦ НУБіП України, 2019) Mazurkevych T.A.; Khomych V.T.The aim of the study was to determine the structure and topography of lymphoid tissue in immune formations of the intestines (Peyer’s patches, Meckel’s diverticulum, apical diverticula) in the postnatal period of ducks’ ontogenesis. Established that lymphoid tissue in these organs located in the tunica mucosa and tunica muscularis of the intestines’ wall. From the one-day age of duck, the lymphoid tissue is found in the tunica mucosa of all studied structures and in the tunica muscularis of the apical diverticula, and in the tunica muscularis from 10–20-days of age in the location of Peyer’s patches and the Meckel’s diverticulum. Lymphoid tissue has three levels of structural organization. These are diffuse lymphoid tissue, primary lymphoid nodules and secondary lymphoid nodules. The presence of all levels of the structural organization of lymphoid tissue indicates its complete morphofunctional maturity. The complete morphofunctional maturity of the mucosal lymphoid tissue of Peyer's patches is reached on the 15 day of ducks’ age, the mucosal lymphoid tissue of the Meckel diverticulum on the 20-day of age, and in the apical diverticula on the 10- day of age. The complete morphofunctional maturity of the muscular lymphoid tissue of Peyer's patches is reached on the 15–25 days of ducks’ age, the muscular lymphoid tissue of the Meckel diverticulum on the 25-day of age, and in the apical diverticula on the 10-day of age. The present article provides the first time data summarizing the structure and topography of lymphoid tissue in the immune formations of the intestines, such as Peyer’s patches, Meckel’s diverticulum and apical diverticula of the Blahovarsky cross broiler ducks.Документ Functional activity and morphological peculiarities of mesenchimal stem cells during in vitro cultivation conditions(ВЦ НУБіП України, 2018) Kladnytska L.V.; Mazurkevych A.I.; Khomych V.T.; Mazurkevych T.A.; Stegney Z.G.; Maluk М.О.; Garmanchuk L.V.; Velychko S.V.; Kharkevych Y.O.; Shelest D.V.; Velychko V.S.; Stupak В.The studies were conducted on 2-3-months-old males of C57BL/6 mice weighing 20-24 g. Obtaining and cultivating of mesenchimal stem cells (MSCs) were carried out in a sterile laminar box with compliance of conditions of asepsis and antiseptics. MSCs of the 2, 4, 7 and 12 passages were analyzed. Morphometric analysis was performed using a light microscopy. Morphometric parameters such as cell and nucleus area or nuclear-cytoplasmic ratio (NCR) were calculated using the Axiovision light microscope (Carl Zeiss, Germany) and ImageJ 1.45 software. Trypan blue dye used for investigation of the viability of MSC. The morphological features of cells during cultivation changes: at first cells have a spindle-like shape with two long cytoplasmic processes, located bipolar. In later passages, cells have a significant number of cytoplasm processes, bipolar arrangement of processes changes to stellar. The NCR index of MSC significant decreases at the 4 passage by 12,9 % (p ≤ 0,05), at the 7 passage - by 35,3 % (p <0,001), at the 12 passage - by 76,6 % (p <0,001) compared to the initial state. The proliferative activity of the MSC of the bone marrow during cultivation significantly dereases at the later passages. Cell resistance to apoptosis induced by cultivation in the serum-free medium is fairly high. The number of cells in the state of apoptosis was 14,0±1,74 at the 4 passage and was reliably increased at the 12 passage to 22,67±1,55 % (p ≤ 0,05) during cultivation.