Перегляд за Автор "Brovarskyi, V.D."

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Histological research of drones’ sperm migration in the reproductive tracts of queen bees
(НУБіП України, 2019) Brovarskyi, V.D.
The research studies sperm migration of drones labeled with fluorescent membrane probes in the reproductive system of bee uterus under various variants of its artificial introduction. It is established that individual doses of sperm of different drones when introduced into the fallopian tubes of the uterus, migrating into the spermateca are placed in layers without significant mixing. It has been found that in odd and even oviducts, sperm lose mobility. On the contrary, with the introduction of sperm into the area of the vaginal opening of the bee uterus, the sperm continue to actively move. It has been proven that during the physiological function of sperm leakage, spermateca from the various portions in the area adjacent to the family line are blotted for egg contamination. The introduction of semen into the area of the vaginal opening with a single portion of several drones helps to mix it in the process of migration in the fallopian tubes and sperm. On the contrary, with the unique introduction of fluorescent membrane probes labeled with doses of sperm of drones at certain intervals, it is possible to direct the reproductive activity of the uterus to obtain predominantly homogenous offspring of parental origin. In this case, the introduction of the sperm of different drones are placed in the spermateca of the uterus in layers, without significant mixing.